WebIn biochemistry and molecular biology, a pulse-chase analysis is a method for examining a cellular process occurring over time by successively exposing the cells to a labeled compound (pulse) and then to the same compound in an unlabeled form (chase). [1] Mechanism [ edit] WebHere, we describe a novel pulse-chase method to determine the half-life of cellular proteins that overcomes the limitations of other commonly used approaches. This method takes advantage of pulse-labeling of nascent proteins in living cells with the bioorthogonal amino acid L-azidohomoalanine (AHA) that is compatible with click chemistry-based ...

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WebPulse-chase analysis to measure protein degradation In order to measure protein half-life by pulse-chase analysis, cells are incubated with labeled precursors, [(35)S]-methionine and [(35)S]-cysteine, for a short time (pulse), after which they are washed from the labeled compound and incubated with excess cold precursor (chase). Web@article{Morey2024SPAACPA, title={SPAAC Pulse-Chase: A Novel Click Chemistry-Based Method to Determine the Half-Life of Cellular Proteins}, author={Trevor M. Morey and … new clover bjd

SPAAC Pulse-Chase: A Novel Click Chemistry-Based Method to …

Web28. feb 2012 · Since IAM is compatible with SPAAC, a more specific azide-dependent labeling is achieved by preincubating proteins containing reduced cysteines with IAM. Supporting Information 1 H NMR spectra of competition experiments between benzyl azide and GSH with either BCN or DIBAC. 1 H NMR spectra of DIBAC incubated with excess GSH. WebSerbo-Croatian: ·smoker ... Definition from Wiktionary, the free dictionary Web20. aug 2024 · Europe PMC is an archive of life sciences journal literature. new cloud jeffersonville indiana