WebThe iScript Select cDNA synthesis kit is a sensitive, flexible, and easy-to-use kit for the generation of first-strand cDNA. This robust reverse transcription kit allows a selection of WebNext, RNA was reverse transcribed into cDNA using iSCRIPT reverse transcription super mix (Biorad). RT-PCR was performed using Power SYBR-green master mix (Life Technologies) with primers for the amplification of the target gene at a concentration of 200 nM. Reactions were carried out in triplicate with 500 nM per primer with a total reaction ...
qPCR/Real-Time PCR Reagents Bio-Rad Two-step Quantitative …
WebBio-Rad iScript Qiagen QuantiTect NEB ProtoScript Bioline SensiFAST SuperScript IV SuperScript III Takara PrimeScript Bio-Rad iScript Qiagen QuantiTect NEB ProtoScript Bioline SensiFAST. Synthesis Kit for target WRKY TF70 (1 ng RNA input) was undetermined (C t ≥40), therefore no standard deviation was WebFeatures and Benefits. Effective gDNA removal in purified RNA samples. Proprietary DNase buffer for maintaining RNA integrity during gDNA removal. Convenient all-in-one, single-tube RT supermix. Ready-to-use no-RT control supermix for monitoring gDNA contamination. gDNA-free cDNA ready in 36 min. opening to joyful noise dvd
iScript Reverse Transcription Supermix for RT-qPCR from Bio-Rad ...
WebBio-Rad Products. Back. Bio-Rad Products Explore all. group1. Biotechnology Laboratory Textbook; AP Biology; ThINQ! Inquiry-Based Kits; ... The iScript cDNA synthesis kit is a … WebJan 4, 2024 · Subsequently, 5 µL of iScript master‐mix was added to 15 µL of RNA suspension, consisting of a volume equivalent to 200 ng of sample RNA mixed with RNase‐free water. The samples were loaded into a thermal cycler (BioRad, T100) using the iScript protocol (5 min at 25 °C, 30 min at 42 °C, 5 min at 85 °C and hold at 4 °C). WebApr 13, 2024 · Total RNA was isolated from orbital fat specimens using the iScript™ cDNA Synthesis Kit (Bio-rad, Inc., Hercules, CA, USA) following the manufacturer’s protocol. Complementary DNA was synthesized using 1 μg of total RNA incubated with random hexamers, 4 μL of 5x iScript Reaction Mix with 1 μL of iScript Reverse Transcriptase, and ... ipac camp pendleton customer service