WebApr 8, 2024 · The cells were fixed with 3% glutaraldehyde and 1% osmium tetroxide, and after dehydration step by step with acetone, the samples were embedded in Epon 812 resin and cut into 50-nm-thick slices.... WebImages of the Western blotting analysis in Figure 5B SFRP1 35KDa β-tubulin 53KDa ker 1 1 2 2 3 3 β-actin 41KDa CRYAA 18KDa ker 1 1 2 2 3 3. P13K ß-tubulin . AKT GAPDH . p-AKT . GJAS GAPDH CRYGS p-actl_n GAPDH . C SFRP ß-tubulin CRY" Title: PowerPoint 演示文稿 Author: 张 诗博 Created Date:

Use Cell-Based Assays to Detect Immunofluorescence in 384-Well …

WebJan 14, 2008 · Gel Electrophoresis and Western Blot Analysis. Cells were incubated for 24 hours in the presence of indicated concentrations of jaceosidin or quercetin. After the … WebApr 13, 2024 · Proteins extracted from the hemolymph EVs were analyzed by 4D label-free proteomics. The EVs were also examined by western blot and immuno-electron microscopy analysis. Intracellular incorporation of PHK26-labeled EVs was tested by adding labeled EVs to tick salivary glands and ovaries, followed by fluorescence microscopy. Results can-am maverick x3 glass windshield

Proteomic analysis of extracellular vesicles from tick hemolymph …

WebFind information on protein visualization and quantitation methods, gel and blot imaging instrumentation, and image analysis software. In-Cell Western Assays. In-Cell Western Assays are high throughput quantitative and … WebCells were plated in 96-well plates at a density of 2,500 per well (100 μL) and cultured in growth medium. The number of the cells was counted according to the protocol of the kit from the company. Western blot Western blot analysis was performed according to the standard protocols. WebThe In-Cell Western Assay is an efficient, time-saving method ideal for those who have already validated the specificity of their antibodies with traditional western blotting or for … can am maverick x3 max doors