WebChlorophyll, starch, and sugar contents are good indicators of growth vigor in plants. To measure the chlorophyll content, we used a modified protocol 1,2. The starch content was determined using iodine staining 3 and enzymatic reactions 4-6. WebNov 23, 2008 · Gene expression, chlorophyll and starch assays were performed when the mutant plants were about 3–4 weeks old and had 6–8 true leaves under 16/8 h of …
Scientific Protocols - Chlorophyll and starch assays - Science …
WebFeb 24, 2015 · Autofluorescence of chlorophyll was monitored using an argon laser at 514 nm for excitation, and emitted light was captured between 662 to 721 nm. Images were processed using ImageJ ... S6 … WebChlorophyll plays an important role in the absorption of light energy of the plant. Chlorophyll can be extracted with a range of solvents. After extraction of the chlorophyll pigments with 80% acetone or 100% methanol, they can be measured in the spectrophotometer. death to spinach
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WebTo prove the necessity of chlorophyll in photosynthesis following steps need to do. Selection of plants with variegated leaves (some leaves are green and some are non-green). Destarching of leaves. A destarched plant is exposed to the sun. A leaf is plucked after a few hours. After marking an outline of the green and non-green part of the leaf ... WebChlorophyll and starch assays . Plants. scientificprotocols authored almost 8 years ago Protocol; Discussion; There are currently no comments on this protocol. Please sign in to participate in this discussion. Back Revisions Embed URL. Average rating 0 ratings. WebThe starch content reached 19.26% (DW) by the 3rd day and finally reached 46.18% (DW) after ABA treatment on the 14th day. ... Effect of ABA on L. punctata chlorophyll content and photosynthesis. ... ether. The eluate was dried down by pure N 2 at 20 °C, and then stored at −20 °C until use for endogenous hormone assay. death to spies moment of truth pc